Today, Hu Ding Xiaobian targeted ELISA If the "whiteboard" phenomenon occurs during the test, what may be the cause and the corresponding solution are summarized as follows: Whiteboard: After the color development step, all the wells of the microplate have no color. Cause Analysis solution Reagents have expired, mixed with different batches Check reagent components and batch numbers to confirm whether they are out of date. Different batch numbers cannot be mixed. Wrong addition, missing substrate, detection A or B Strictly follow the experimental steps to observe whether the liquid level is consistent and reduce leakage Enzyme label is contaminated and inactivated during washing or loading Confirmation test B buffer Contains no enzyme inhibitor, confirm that the preparation container is clean There is a problem with distilled water Confirm that the purified water for the preparation of the wash solution meets the requirements and is not contaminated, compared with good distilled water. Concentrated type for washing liquid, no dilution before washing The washing liquid should be diluted according to the experimental requirements Enzyme conjugate activity is low or has been inactivated Enzyme conjugates are tested at regular intervals to determine the activity of the enzyme conjugate Solution PH Incorrect value Guaranteed dilution PH Value in physiological conditions PH Near the value, ie 7.2-7.4 about
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